January 06, 2016

Genetic doping can permanently increase the muscle mass in mice and there are delivery methods which could avoid the need for immune system suppression

The origins of genetic doping have nothing to do with sports. Rather, researchers have been trying to develop ways to repair muscles in people with muscular disorders. Here's how it works: A synthetic gene is engineered to secrete a specific protein, one that's normally involved in muscle growth and repair. That gene is delivered by an otherwise harmless virus, and when it reaches the cell it's designed to work with, it "turns on." With access to more of the protein than would normally be produced, the damaged muscle is enhanced. Current techniques allow this all to happen without actually altering a person's genetic makeup.

Highlights
  • Gene therapy has added 15% more muscle mass in monkeys and the effect has lasted for 15 years
  • There are ways to circumvent the need for viruses to deliver gene therapy
  • Viral gene therapy delivery requires temporary immune system suppression
  • Arrays of microneedles can directly deliver treatment to muscles
  • Gene therapy can be enclosed in tiny bubbles of fat

Details

According to Dr. H. Lee Sweeney, a professor of physiology at the University of Pennsylvania Medical School who's worked to develop such treatments, healthy athletes could benefit greatly from similar methods. "The same things, if introduced into normal muscle, would make them much more responsive to exercise and training, and much more responsive to repairing themselves following an injury," says Sweeney. For that reason, Sweeney doesn't believe sports leagues and governing bodies will allow it.

The change in muscle performance for an elite athlete could be substantial. The actual effect would depend on a number of factors (including the intensity of training), but in tests, lab rats who were injected and then made to do resistance exercises increased their muscle mass by 15 percent on top of what they would have normally achieved with exercise alone. More important to an athlete, the effects could last for years, if not decades. Researchers tested it on monkeys some 15 years ago and still haven't seen the induced changes drop off.

Dr. Charles Yesalis, a professor emeritus at Penn State who's researched performance-enhancing drugs, says he expects this "cascade" method of doping — in which athletes trick their bodies into releasing more of something that would increase performance — will become increasingly common as new advances are made. And while he says there are different ways athletes could use such a method, "The No. 1 thing that comes to mind is genetic doping."


Photo: Joseph McNally/Getty Images



The medical advancements required to make these techniques widespread have come slowly, but progress is being made. Trials are under way for a localized procedure that would allow doctors to target specific muscles with simple injections. And while access to these viruses is limited at the moment, if an athlete could find a rogue scientist to provide him with these injections, in theory he or she could be using them right now. (A related method of doping, using antibodies to block molecules that interfere with muscular growth and repair, is even closer to approval.)

But in the future, instead of injecting genes into particular muscles, doctors will likely be able to deliver them to the entire body intravenously, thereby allowing an athlete to amplify the effect of training on every single muscle he or she works out. This would also allow athletes to bounce back from injuries faster — and do so in a way that would permanently enhance their muscles.

Sweeney says we're at least a decade away from such a technique, because in order for it to work, the athlete's immune system would need to be suppressed, so the body doesn't try to fight off what it identifies as a virus. It's a dangerous process that will take a long time to perfect, and it has yet to be tested on adult humans. Sweeney estimates that the earliest of those trials will take place over the next five years, and any timetable would depend on how well those trials go. "The timeline between now and when these would be available could be anywhere from ten years to who knows," says Sweeney.

NBF - The timeline could be shortened if other means applying gene therapy did not involve viruses that require immune system modification.

Gene Therapy delivered in bubbles of fat to lungs via a nebuliser

Cystic fibrosis is a genetic condition caused by a mutated gene called CFTR. The mutation causes the lungs and digestive system to become clogged up with sticky mucus.

The goal of gene therapy for cystic fibrosis is to replace the faulty CFTR gene with a working one.

Previous attempts of using a virus to deliver the working gene proved unsuccessful, as the lungs' defense system against infection stopped the virus from entering.

In this new study, the researchers tried a different approach – the gene was encased in a bubble of fat, which was then delivered to the lungs via a nebuliser.

When compared to placebo, the nebuliser-delivered approach showed a modest, but significant, improvement in lung function (3.7%)

Micro Nano delivery through the skin and into muscle

Patches with arrays of microneedles can be used to get through the skin to deliver therapy to the muscles. The microneedles can have nanopatterning which cause the spaces between cells to widen.




Another innovative strategy to overcome the barrier function of the epithelium, and in particular stratified epithelium, is through the use of microneedles. Microneedles pierce the skin in a non-invasive and painless way. They penetrate the outer 10–20 µm of the skin, creating shunts to the dermis for delivering drugs topically or systemically. Microneedles are fabricated with a wide range of materials and are typically fabricated as an array of up to hundreds of microneedles over a substrate. The first microneedles were produced from silicon wafers by photolithography followed by deep reactive ion etching.24 Other production methods have recently been developed for creating less expensive and biocompatible materials such as metal, polymer, and sugar-based microneedles. Metal microneedles are mainly produced through laser cutting from sheet metal and bending them perpendicularly out-of-plane. Polymeric microneedles can be biocompatible and because of their viscoelastic properties, they are less prone to breakage once in the skin. Drugs can also be incorporated into biodegradable polymeric microneedles for controlled delivery.

Finally, to overcome the tight junctional complex of the epithelium, we have recently reported that nanostructured topography loosens the barrier function of epithelial tissue by interacting with the tight junctions. An aligned array of low aspect ratio nanopillars fabricated through NIL is capable of reversibly remodeling the tight junction proteins of simple epithelia. This loosening of the tight junctions allows for paracellular transport of high molecular weight therapeutics that are up to four orders of magnitude greater than the current molecular weight limits of the epithelium. Unlike harsh chemical permeabilizers which oftentimes damage epithelial cells, this nanofabricated approach has limited side effects due to the apparent reversibility in the tight junction remodeling. The ability to deliver high molecular weight biologics without the need for hypodermic needle injections has great potential in the field of drug delivery, and specifically in biotechnology

SOURCES - NY Mag, NHS UK, NCBI Journal of Material Chemistry, University of Pennsylvania Medical School, University of California San Francisco

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