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July 03, 2008

Artificial DNA Made Exclusively of Nonnatural bases made in Japan


Chemical structures and synthesis of artificial DNA. (a) Chemical structures of two types of natural and nonnatural base pairs. (b) Synthetic strategy for the artificial DNA

Japan makes artificial DNA H/T nanowerk and Alfin

This is separate from the GNA that was created glycerol nucleic acid (GNA, —a synthetic analog of DNA). It was made by Biodesign Institute scientist John Chaput and his research team.

It is also different from the two new base letters that were added to regular DNA

To those who don't believe in a Technological Singularity - really ? behind schedule ? no progress ? There just had to be a specification of which artificial DNA breakthrough is being discussed from announcements made this year.

Artificial DNA Made Exclusively of Nonnatural C-Nucleosides with Four Types of Nonnatural Bases

A new class of DNA-like oligomers made exclusively of nonnatural, stable C-nucleosides. The nucleosides comprise four types of nonnatural bases attached to a deoxyribose through an acetylene bond with the β-configuration. The artificial DNA forms right-handed duplexes and triplexes with the complementary artificial DNA. The hybridization occurs spontaneously and sequence-selectively, and the resulting duplexes have thermal stabilities very close to those of natural duplexes. The artificial DNA might be applied to a future extracellular genetic system with information storage and amplifiable abilities.




Artificial DNA made exclusively of nonnatural nucleosides with four types of nonnatural bases represents a new class of DNA-like synthetic oligomers. The iG*/iC*-rich artificial DNA forms right-handed duplexes with the complementary artificial DNA in a sequence-specific manner with antiparallel orientation. The thermal stabilities and thermodynamic parameters of the artificial duplexes are very close to those of the natural duplexes in spite of differences in their geometries and hydrogen-bonding patterns. On the other hand, the artificial homooligomeric DNAs consisted only of A* and T* were found to exclusively form triplexes. By means of the synthetic feasibility of the artificial nucleoside unit, a variety of candidates for nonnatural bases can be incorporated into the artificial DNA. Therefore, the present molecular framework has a potential for storing genetic information and for application to enzymatic replication directed toward engineered genetics. Furthermore, the artificial DNA may be a superior building scaffold for constructing nanostructures of materials interest because of the stable C-nucleosides against ubiquitous naturally occurring enzymes such as DNase.

FURTHER READING
Supporting information on the procedures used for the artificial DNA

Further analysis from Ars Technica

1 comments:

kurt said...

So, when do we get synthetic cells and synthetic stem cells for human regeneration/rejuvenation.

Maybe these guys will come out with the Nexus 6 replicants in time for holiday shopping season in 2016. Of course, limiting them to a 4-year life span is going to really piss them off.